Formation of syncytium upon RSV infection of human and ovine LR-MSCs.
(A, B) Representative micrographs of pediatric (A) and ovine (B) LR-MSCs infected with 0.1 PFU/cell of RSV-mCherry 48–72 hours p.i. Giant multinucleated cells, indicative of syncytium formation, were observed. Yellow arrowheads indicate clusters of nuclei and the yellow dashed line indicates a giant multinucleated cell. PC, phase-contrast. Magnification 100X (PC, pediatric LR-MSCs) and 400X (PC and RSV, ovine LR-MSCs). Scale bar, 125 μm.