Forced recruitment of TET1-CD to PCH and chromocenter clustering.
(A): Schematic illustration of the experimental design. GCN4 was conjugated with catalytically-dead Cas9 (dCas9), which was recruited to PCH by the sgRNA targeting major satellite repeats, and GCN4 was targeted by scFv conjugated with TET1-CD. (B): Schematic illustration of experimental conditions. (C): Representative immunostaining images of the surface-spread nuclei of the ESCs expressing individual cassette. Scale bar, 5 μm. (D): Boxplot showing the number of distinct chromocenters in the nuclei of ESCs expressing individual cassette, related to (C). scFv-only, n = 34; dCas9 control, n = 48; TET1-CD, n = 44; TET1-CD-Mut, n = 41. P values were calculated using the Mann-Whitney U-test. ***P < 0.001.