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Fluorescence emission of the probe bis-ANS in the presence of SP-BN.

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posted on 05.07.2016, 17:36 authored by Ángeles Bañares-Hidalgo, Jesús Pérez-Gil, Pilar Estrada

Fluorescence intensity at 480 nm of the probe (11 μM) in the presence of SP-BN in 5 mM AMT buffer, 150 mM NaCl pH 7 with urea (filled circles, dotted line) and GdmCl (void circles, solid line) vs denaturant concentration.

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Conformational Stability of the NH2-Terminal Propeptide of the Precursor of Pulmonary Surfactant Protein SP-B
PLOS ONEPLOS ONE

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2Osurfactant protein B500 mM NaClPulmonary Surfactant Protein SP-B AssemblyT m 25.7 M GdmClNon-two States modelNHDTNBSP-B Ndisulfide bonds establishmentsurfactant biogenesis pathwaysurfactant lipid-protein complexes150 mM NaClDifferential scanning calorimetry2 M urea

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