pone.0198288.g001.tif (1.97 MB)
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Flow chart outlining the analytic approach for the current study.

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posted on 18.07.2018, 17:27 by Steven D. Hicks, Neil Khurana, Jeremy Williams, Cindy Dowd Greene, Richard Uhlig, Frank A. Middleton

Briefly, sample sets 1 and 2 were used to identify circadian RNA candidates (green), which were then validated in sample set 3 (blue). Relationships between CircaMiR levels and CircaMicrobes, or mRNA targets were explored (orange). The functional implications of CircaMiRs and CircaMicrobes were interrogated through annotation analyses and characterization in a cohort of children with disordered sleep (sample set 4; red). The relationship of oscillating RNA with patterns of daily activity (sleep, eating, and tooth brushing) were also investigated.