Fig. S1.tif (898.79 kB)
Figure S1
Figure S1: Methodology for construction of E. coli hosts expressing the type I restriction modification systems of BPH0662. A schematic representation of the methodology for the construction of E. coli host strains DC10B-MS1 (which expressed the hsdMS1 from S. epidermidis BPH0662) and DC10B-MS2 (which expressed the hsdMS2 from S. epidermidis BPH0662). AmpR = ampicillin resistant; ts = temperature sensitive; KanR = kanamycin resistant.