Figure 6. Sites of full conservation and positive selection on the model A. thaliana CERK1 across non-mycorrhizal and fungal associated species. The template structure of A. thaliana CERK1 was obtained through AlphaFold. LsyM domains (Orange) of Non-Mycorrhizal (A, C) and Fungal-Associated (B, D) species shown against full protein structure (Blue). Conservation visualized in NM/FA species (Cyan), NM: 6, FA: 2. Sites found to be under positive selection (Green) NM: 11, FA: 8. Sites under positive selection were derived from a coarse, 5-nucleotide sliding window analysis through DnaSP6 to observe patterns of selection.