Figure 5. Amino acid frequency probability in each position of the LsyM domain. An amino acid sequence logo was created in RStudio using Non-Mycorrhizal (A) and Fungal-Associated (B) protein sequences which were aligned from CDS sequences using MUSCLE in AliView. Chemistry and frequency of each amino acids was visualized using ggplot2 and ggseqlog scripts. The resulting graph shows acidic (Red), basic (Blue), hydrophobic (Black), neutral (Purple), and polar (Green) residues. Limited conservation was observed with most occurring in polar (Green) and hydrophobic (Black) chemistry.