Fig. S1 and Fig. S2

Fig. S1. LSCM and TEM micrographs of ROIs 3-5 from Figure 2. A) ROI 3 was selected as an additional negative control of EVs. B and C) High (squares 1) and weaker (squares 2) intensity green fluorescence signal areas were analyzed within the selected ROIs 4 (B) and 5 (C). D). High magnifications TEM of panel 1 y 2 correspond to the selected areas within the ROIs. Scale bars in A: ROI 3: 0.5 µm; ROI 4 and 5: 1 µm. In B: ROI 4 and ROI 5: 0.5 µm.

Fig. S2. LSCM and TEM images of BMV virions. A) Low magnification images of BMV virionslabeled with FITC (green signal). The ROI is marked as 1 (white square). B) TEM images from ROI 1 and its sub-ROIs (2-3.1). Scale bars in A: 25 µm, B panel 1: 2.5 µm; panel 2: 0.5 µm; panel 3: 200 nm; and 3.1 inset: 50 nm.