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posted on 2023-05-10, 17:32 authored by Asma Rafique, Amber Afroz, Nadia Zeeshan, Umer Rashid, Muhammad Azmat Ullah Khan, Muhammad Irfan, Waheed Chatha, Muhammad Ramzan Khan, Nazia Rehman(2A) DsRNA feeding experiment for checking the efficacy of lac gene as RNAi target. Effect of artificial diet (20% sucrose), GFP dsRNA (20 ngL-1) -ve control, and Lac gene (LAC) (7 μg/μL) (2B) Fold increase in mRNA expression (determined by of Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) of Lac gene in comparison to internal control (Actin) and -ve control (Lac without dsRNA feeding assay) 2D, 4D and 6D post-feeding. (2C) Fold change in mRNA expression in leaf, stem and root; determined by Lac gene qRT-PCR in comparison to internal control (Actin) 9D, 11D and 13D post-spraying. Means are significantly different at a 0.05 level of significance at α = 0.05.
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wheat biotic stressesphylogenetic analysis displayedmain factors devastatingdiv >< pdesign 143 sirna250 μm acetosyringonetriticum aestivum sitobion avenae agrobacterium tumefaciens russian wheat aphidsgalaxy 2012 yieldedcvs using laccaseachieve lac silencingpotential rnai targetone dsrna targetaphid mortality postmaximum transformation efficiency5 %), followedgalaxy 2012rnai targetvitro planta russian graingrain aphids5 %).13d post8 %),16 %),systemic movementreverse transcriptasepeach potatoobserved 8dlac expressiongg providesfound similarernai toolcopper oxidaseanaj 2017>- resistant3h co2 %).
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