False positive composition.

(A,B) The number of false positive calls in 0.05% allele frequency intervals is shown for ERASE-Seq using 1, 2, 3, and 4 replicates for the amplicon panels 56G and TST15. (C,D) The number of false positives using standard intra-sample variant calling metrics (base-quality, strand-bias and read-depth filters) are shown in 0.05% allele frequency intervals for 56G and TST15. They are further divided into recurrent artifacts and stochastic errors. Stochastic errors are those called in single replicate ERASE-Seq and recurrent artifacts are those eliminated in single replicate ERASE-Seq based on the background model.