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False positive composition.

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posted on 2018-04-09, 17:39 authored by Nick Kamps-Hughes, Andrew McUsic, Laurie Kurihara, Timothy T. Harkins, Prithwish Pal, Claire Ray, Cristian Ionescu-Zanetti

(A,B) The number of false positive calls in 0.05% allele frequency intervals is shown for ERASE-Seq using 1, 2, 3, and 4 replicates for the amplicon panels 56G and TST15. (C,D) The number of false positives using standard intra-sample variant calling metrics (base-quality, strand-bias and read-depth filters) are shown in 0.05% allele frequency intervals for 56G and TST15. They are further divided into recurrent artifacts and stochastic errors. Stochastic errors are those called in single replicate ERASE-Seq and recurrent artifacts are those eliminated in single replicate ERASE-Seq based on the background model.