FGF23 treatment led to changes in renal NaPi-IIa and Vdr protein expression.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Representative western blots of total renal protein homogenates from mice injected with either vehicle (Ctrl) or recombinant human FGF23 (rhFGF23) once a day for two consecutive days using antibodies against NaPi-IIa (A), full length Klotho (B), Cyp24a1 (C) and Vdr (D). The proteins of interest are shown in the top panels, whereas the corresponding β-actin signal is shown in the bottom image. Densitometric quantification was normalized to the corresponding β-actin values. Bar graphs represent mean ± SD (n = 5-6/group) of the normalized values and data was analyzed by unpaired student’s t-test with significant p values indicated as: * p ≤ 0.05.