FACS analysis of splenic B2 B cells in uninfected and T. brucei ANTat 1.1-infected C57BL/6 and Prf1-/- C57BL/6 mice.
(A) Transitional B cells (IgM+AA4.1+B220+) in a representative uninfected C57BL/6 mouse, (B) Transitional B cells in representative day 10 T. brucei infected C57BL/6 mouse; (C)–Marginal zone B cells (IgM+CD21+CD23lo) and follicular B cells (IgM+CD21+CD23hi) in a representative uninfected C57BL/6 mouse, (D) Marginal zone B cells and follicular B cells in a representative day 10 T. brucei infected C57BL/6 mice; (E)—Transitional B cells in a representative uninfected Prf1-/- C57BL/6 mouse, (F) Transitional B cells in a representative day 10 T. brucei infected Prf1-/- C57BL/6 mouse; G Marginal zone B cells and follicular B cells in a representative uninfected Prf1-/- C57BL/6 mouse, (H) Marginal zone B cells and follicular B cells in a representative day 10 T. brucei infected Prf1-/- C57BL/6 mice.