Extensive treatment with CMCT alters the processing of U3 and tRNALeu in S. cerevisiae.
(A) Northern blot analysis of the U3 and tRNALeu processing. Reaction conditions at two timepoints are specified at the top of the figure, and concentrations of CMCT incubation are listed below reaction time conditions. scR1 RNA is the loading control. (B) The quantification of the data in panel A. Graphs represent two independent experiments. Significance was determined using a t test; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; n.s., nonsignificant.