Expression of PvTET1A, PvTET3, PvTET4, and PvTET8 in P. vulgaris during nodule development.
Transcript accumulation is observed during nodule development under rhizobia colonization. (A) PvTET1A, (B) PvTET3 and (C) PvENOD40 transcript accumulation after Nod factor (NF) treatment or inoculation with R. Tropici CIAT 899 are depicted. PvTET4 (D) and PvTET8 (E) specifically respond to rhizobia inoculation. These results are compared with uninoculated roots harvested at the same time (green bars). Expression values were normalized with those of EF1a. Bars represent means ± SEM of at least three independent biological replicates with three technical repeats. P-values <0.05 are marked with two asterisks (Student’s t-test). Promotor activity of PvTET8 in lateral root primordia (F) and nodule primordia development (G).