Expression of CBF and COR genes in rbd1-1 mutant.
(A-B) Expression level of CBF1, CBF2 and CBF3 (A) and COR15A, KIN1 and COR47 (B) after 4°C treatment at different time points. RNAs were isolated from five newly emerging leaves from 2 weeks old plants grown at normal condition followed by 4°C treatment. The Actin gene was used as a normalization control and the experiments were repeated three times with similar results (A). Equal loading was controlled by cytosolic 25S rRNA (25S) stained with ethidium bromide (B). (C) The expression patterns of RBD1 under 4°C. Total RNA was extracted from two weeks old plants chilling-treated for 3, 7, 14 and 21 days. The Actin gene was used as a normalization control. The experiments were repeated three times with similar results.