Figure S1 - Expression at the Imprinted Dlk1-Gtl2 Locus Is Regulated by Proneural Genes in the Developing Telencephalon

Expression of Gtl2 RNA (ISH) at E17.5 in Ngn2 WT and Ngn2 KO mice. (A-E) Co-labeling with Tbr1 protein (immunohistochemistry) was used to show specific localization of Gtl2 positive neurons in the dorsal telencephalon (VZ = ventricular zone, IZ = intermediate zone, CP = cortical plate). Cells that show ectopic expression of Gtl2 mRNA in Ngn2 KO mice are localized in the IZ (red arrows in B and D). Scale bars: 150 µm.

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