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Expression and purification of GST-L1-E7 protein in E. coli system.

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posted on 2021-10-14, 17:37 authored by Elnaz Abbasifarid, Azam Bolhassani, Shiva Irani, Fattah Sotoodehnejadnematalahi

A) SDS-PAGE analysis: lane 1: Molecular weight (10–140 kDa, Fermentas), lane 2: Before induction, lane 3: 3 h after induction, and lane 4: Purified protein; B) Identification of the recombinant GST-L1-E7 protein by western blot analysis using anti-His antibody: lane 1: Molecular weight, lane 2: Before induction, lane 3: After induction, and lane 4: Purified protein.

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