Expression and distribution of cell vesicles positive for Lamp-2 or cathepsin D in untreated and Retro-2-treated macrophage-like RAW 264.7 cells.
Representative 3D reconstruction CLSM micrograph showing the unchanged cytoplasmic localization of Lamp-2- and cathepsin D (CatD)-positive vesicles in Retro-2(1 μM) -treated cells compared to untreated cells. To the right of the micrograph, bar graphs showing quantification of the Lamp-2 or CatD relative fluorescence intensity (RFI) in untreated and Retro-2-treated cells. CLSM micrographs are representative of two independent experiments. Values represent the average (± SEM) obtained by examining at least 30 infected cells per condition.