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Expression analysis of proteins in the PI3K/Akt/mTOR signaling pathway in glioma and GBM samples.

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posted on 09.04.2014, 03:55 authored by Katrin Höland, Danielle Boller, Christian Hagel, Silvia Dolski, András Treszl, Olivier E. Pardo, Paulina Ćwiek, Fabiana Salm, Zaira Leni, Peter R. Shepherd, Beata Styp-Rekowska, Valentin Djonov, André O. von Bueren, Karl Frei, Alexandre Arcaro

(A) IHC staining of glioma tissue microarray demonstrating increased expression of all antigens shown in GBM (WHO grade IV) compared to pilocytic astrocytoma (WHO grade I). Bars represent 100 μm. (B) Multivariable Cox-regression of IHC factors in 74 glioma patients by individually adding the immunostaining status to the clinical factors age, gender, and WHO grade. Results for p-S6 (S235/236) and S6 are shown in the upper and lower panel, respectively. HR: hazard ratio, LCL/UCL: lower/upper boundary of 95% confidence interval for HR. (C) Western blot analysis of protein levels of EGFR, PTEN, class IA PI3K isoforms, and downstream signaling proteins Akt and S6 in human GBM cell lines and ex vivo cultures. Normal human brain and cerebellum tissue as well as non-transformed type II human pneumocytes were used as controls.