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Experimental design of the study.

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posted on 04.04.2016, 12:57 by Sara Nullens, Michael Staessens, Cédric Peleman, Philip Plaeke, Surbhi Malhotra-Kumar, Sven Francque, Joris G. De Man, Benedicte Y. De Winter

In a first experimental setup (A), mice received anti-interleukin-6 antibodies (1 mg/kg) or the vehicle (IgG1 kappa isotype) simultaneously with the CLP- or sham-procedure. In a second setup (B), mice furthermore received a repeated injection with the antibodies 24h following the procedure. In a third setup (C), mice only received anti-IL-6 or vehicle 24h following the CLP- or sham-procedure. 48h post-CLP, mice received a gavage of colored solid beads and 2h later mice were sacrificed for measurement gastrointestinal transit, prelevation of serum and tissue samples, measurement of colonic permeability and quantification of cell adhesion molecules at the mRNA and protein level. CBA: cytometric bead array; CDS: clinical disease score; CLP: caecal ligation and puncture; MLN: mesenteric lymph nodes; mRNA: messenger ribonucleic acid; RT-PCR: reverse transcriptase real-time polymerase chain reaction.