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Experimental design.

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posted on 16.01.2020, 23:06 by Gangling Chen, Pradip Kumar Kamat, Abdullah Shafique Ahmad, Sylvain Doré

In cohort 1 (Iso MCAO + iso sham LRIP), mice underwent MCAO surgery under isoflurane anesthesia (Fig 1). At 90 min after reperfusion, sham-LRIP was induced under isoflurane anesthesia. In cohort 2 (Iso MCAO + iso LRIP), mice underwent MCAO surgery under isoflurane anesthesia. At 90 min after reperfusion, LRIP was induced under isoflurane anesthesia. In cohort 3 (Hal MCAO + k-x sham LRIP), mice underwent MCAO surgery under halothane anesthesia. At 90 min after reperfusion, sham-LRIP was induced under ketamine-xylazine anesthesia. In cohort 4 (Hal MCAO + k-x LRIP), mice underwent MCAO surgery under halothane anesthesia. At 90 min after reperfusion, LRIP was induced under ketamine-xylazine anesthesia. In cohorts 2 and 4, LRIP was induced at 90 min after reperfusion by three cycles of 5 min ischemia/5 min reperfusion of bilateral hind limb for one session per day for a total of four sessions.

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