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Exogenous HIV-1 VLPs colocalize with Siglec-1 and p24 in the VCC of HIV-1 BaL-infected MDMs.

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posted on 27.01.2017, 04:30 by Jason E. Hammonds, Neal Beeman, Lingmei Ding, Sarah Takushi, Ashwanth C. Francis, Jaang-Jiun Wang, Gregory B. Melikyan, Paul Spearman

(A-B) MDMs were infected with primary HIV-1 isolate BaL at a TCID50 of 0.5/cell. On day 9 post-infection 400 ng of exogenous HIV-1 Gag-EGFP VLPs were added to the culture overnight. On day 10, MDMs were washed, fixed in 4% paraformaldehyde and immunostained for p24 (red), Siglec-1 (magenta) and DAPI co-stained. (C-D) MDMs were infected and exogenous HIV-1 VLPs added as described in (A-B). MDMs were washed, fixed in 4% PFA and immunostained for p24 (red), CD9 (magenta) and DAPI co-stained. Size bars = 10 μm.