Exclusion of one biological replicate from the analysis due to outlier status.

Multivariate analysis based on the expression profiles of the 500 genes with the highest variance across all samples showed that one biological replicate corresponding to females courted by related males (2 empty circles representing 2 technical replicates) is far from the other points (full circles) A) in the plan defined by the two first axes of the principal component analysis and B) in the sample hierarchical clustering dendrogram. Significant statistical support of outlier status is indicated by an approximately unbiased p-value with one million multiscale bootstrap replicates.

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