Example of Nanoparticle tracking analysis (NTA).
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posted on 2023-03-27, 17:37 authored by Xuesheng Wu, Maite Goebbels, Lemeng Chao, Tom Wennekes, Frank J. M. van Kuppeveld, Erik de Vries, Cornelis A. M. de HaanAn example of SeV concentration analysis using the NanoSight NS300 instrument is shown. The black line corresponds to the average virion concentration (5 measurements), while the red curve represents the standard deviation. Particle concentration (in 1010 particles/ml) and diameter (in nm) are graphed on the Y- and X-axis, respectively.
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used biolayer interferometrymay immobilize virionsperform kinetic analysesvirions cleaved sialoglycansnewcastle disease virusfunctional decoy receptorsdriven virion releasedriven virion motilitythreshold receptor densityparamyxoviruses display sialidasedriven releasevirions startvirion concentrationspecific densitysimilar motilitysialoglycans evirion bindingzoonotic potentialspecies tropismsheer abundancesendai virusreceptors comesreceptor interactionsoften presentlargely independentkinetic analysiskey determinantsinteracting embecoviruseshuman paramyxovirusesfurthermore showndynamic interactionscooperative processcoated surfacecleavage increasescleavage activitiescell surfacealso apply
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