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Evolved tetraploids can stably maintain their genomes when growing under non-optimal conditions.

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posted on 2016-11-03, 17:48 authored by Yi-Jin Lu, Krishna B. S. Swamy, Jun-Yi Leu

Eight ancestral tetraploid clones (anc4N-1 to anc4N-8) and eight evolved tetraploid clones (evo4N-1 to evo4N-8) were propagated in regular YPD medium at 30°C for 200 generations and the evolved cultures were analyzed using flow cytometry. Half of the cell lines derived from ancestral clones became aneuploid or diploid, but all cell lines derived from evolved clones remained tetraploid.