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Evaluation of the role of NLRP3 inflammasome activation in STSLS with MCC950.

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posted on 06.06.2019, 17:55 by Lan Lin, Lei Xu, Weihua Lv, Li Han, Yaozu Xiang, Lei Fu, Meilin Jin, Rui Zhou, Huanchun Chen, Anding Zhang

Mice were infected with S. suis epidemic strain SC-19 and then treated with MCC950 (NLRP3 inhibitor) or control to evaluate the role of NLRP3 in STSLS. (A) Cytokine levels in peritoneal lavage fluids at 6 h post-infection were determined using ELISA kits (two-tailed, unpaired t-tests, n = 5). (B) Blood levels of AST, ALT, LDH and CK at 6 h post-infection (two-tailed, unpaired t-tests, n = 5). (C) H&E staining of infected tissue sections from mice at 6 h post-infection with S. suis epidemic strain SC-19 with or without MCC950 treatment. Congestion in the lung and spleen is indicated by a “red arrow”, infiltration of inflammatory cells in the lung is indicated by a “hollow arrow”, vacuolated degeneration in the liver is indicated by a “black arrow”, and necrosis in the liver is indicated by a “yellow arrow”. (D) Clinical symptom scores of mice infected with S. suis epidemic strain SC-19 and treated with or without the NLRP3 inhibitor MCC950 (two-way RM ANOVA, n = 10). (E) Survival of mice infected with S. suis epidemic strain SC-19 and treated with or without the NLRP3 inhibitor MCC950 (log-rank test, n = 10). (F) The bacterial load in the blood at 6 h post-infection was determined to evaluate the role of NLRP3 in S. suis clearance (two-tailed, unpaired t-tests, n = 5). Error bars represented the mean ± standard deviations.

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