Establishing the fluorescence polarization method to study IMP1-RNA interaction.
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posted on 2020-05-07, 08:57 authored by Almas Yaqoob, Wai Ming Li, Victor Liu, Chuyi Wang, Sebastian Mackedenski, Linda E. Tackaberry, Hugues B. Massicotte, Keith N. Egger, Kerry Reimer, Chow H. LeeFluorescence polarization analysis of the interaction between the wild-type IMP1 and its point-mutation variants (KH1-2, KH3-4 and KH3) with the fluorescein-labeled 39-nt CD44 (A) and the 44-nt KRAS RNAs (B). Results shown are representative of three separate experiments (n = 3).
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KRAS mRNA expressionIMP 1colon cancer cell linesoncogenic insulin-like growth factor 2 mRNA-binding protein 1ethanol extractscolon cancer cellsdown-regulating KRAS expressionfluorescence polarization assaypolypore Albatrellus flettiiterricolous polypore Albatrellus flettiiKH 4 domaincontrol antibiotic neomycinIMP 1.anti-cell viability componentsanti-cell viability activitySWdi-domain KH 3KRAS RNAconfluentinIMP 1-KRAS RNA interaction
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