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Endoreplicating SCs have larger nuclei and greater biosynthetic secretory activity than other SCs.

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posted on 07.10.2019, 17:33 by Aaron Leiblich, Josephine E. E. U. Hellberg, Aashika Sekar, Carina Gandy, Claudia C. Mendes, Siamak Redhai, John Mason, Mark Wainwright, Pauline Marie, Deborah C. I. Goberdhan, Freddie C. Hamdy, Clive Wilson

(A) Histogram showing SC:MC nuclear size ratio for EdU-positive and EdU-negative SCs from esgtsF/O control mated males versus virgins. (B) Confocal microscopy image of males after a 24-hour pulse of CD63-GFP expression in SCs directly after multiple matings. Some SCs are marked by dashed circles (red for SCs with adult endoreplication, white for those without; number of CD63-GFP-marked compartments is given in each case). Not all labelled compartments can be seen in this single z-plane. Magnified maximum projection image shows examples of one EdU-positive and one EdU-negative SC, with each labelled compartment marked with a red or white asterisk, respectively. (C) Histogram showing number of CD63-GFP-positive compartments per cell in EdU-positive and EdU-negative SCs from multiply-mated males, following a 24-hour pulse of CD63-GFP expression. Data analysed by one-way ANOVA with Sidak’s multiple-comparisons test (A) or a two-tailed Mann-Whitney test (C). **p < 0.01, ***p < 0.001, ****p < 0.0001, n ≥ 21 (A), n ≥ 29 (C). Scale bars, 20 μm. Underlying data for this figure can be found in S1 Data. EdU, 5-ethynyl-2′-deoxyuridine; esg, escargot; esgtsF/O, the yeast transcription factor GAL4 expressed under the control of the promoter of the gene esg in a temperature-dependent fashion; GFP, green fluorescent protein; MC, main cell; No., number; SC, secondary cell.