Endocytosis and transcytosis of nanocarriers with 20nt or 40nt dsDNA cargo.
(A) Uptake of NP-DNA-20ds and NP-DNA-40ds into brain endothelial cells. Nanoparticles were quantified in the cytosol, vesicles, nucleus, and at the basal membrane (undercell). NP-Gal was the nanoparticle control, no DNA attached. Three experiments were performed, each experiment having three technical repeats. Tukey's multiple comparisons test showed significant difference for NP-DNA-40ds compared to NP-DNA-20ds in cytosol (*** P = 0.0003), vesicles (** P = 0.0082) and under cell (* P = 0.0108). (B) Electron micrographs of silver enhanced gold NPs in vesicles and at the basal membrane of hCMEC/D3 cells treated at the apical membrane (top) with NP-DNA-40ds (A) or NP-DNA-20ds.