Elevated expression of fimD in bipA-deleted strains.
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posted on 2025-04-09, 17:40 authored by Eunsil Choi, Eunwoo Ryu, Donghwee Kim, Ji-Won Byun, Kahyun Kim, Minho Lee, Jihwan HwangTotal RNA was extracted from wild-type and bipA-deleted strains in the early exponential phase at 37°C or 20°C and analyzed using qRT-PCR. The relative expression levels of the type 1 fimbriae gene fimD were normalized to the endogenous control gene rrsA.
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surface structure biogenesisstress conditions relevantprevious research showeddiv >< pcompromising membrane integritybacterial fitness factor37 ° c20 ° chighly conserved protein50s ribosomal assemblysalmonella enterica escherichia coli attenuated strain lt2typhimurium strains revealedlps defect activatesribosome assemblyincreasing proteintyphimurium strainsstrains exhibitedtwo pathogenictranslational gtpasesstructurally similarserovar typhimuriumrelated processespresumably activatingnegative bacteriamouse modelmediated motilityinvasion capabilityimpaired lipopolysaccharidehct116 ).functional attenuationfindings suggestdual functionsdeletion mutantsdecreased flagellacapsular polysaccharidesbipa biofilm formation
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