Efficient R-induced lytic reactivation requires expression of YAP and TAZ.
A) NOKS-Akata cells were transfected with control siRNA or siRNA against YAP or TAZ, and then 24 hours later cells were transfected with an R expression vector or control vector. After another 24 hours an immunoblot was performed to assess the levels of R, YAP, TAZ, Z, BMRF1, and HSP90. B) NOKS-Akata cells were transfected with control siRNA or siRNA against YAP, and 24 hours later were transfected with both Z and R expression vectors. A western blot was performed to determine expression levels of YAP, Z, R, BMRF1 and tubulin. C) NOKS-Akata cells were transfected with control siRNA or siRNAs against TAZ, and were then transfected 24 hours later with Z and R expression vectors. The cells were harvested after 24 hours for western blot analysis to detect expression of TAZ, R, Z, BMRF1, and tubulin. Results were quantitated using ImageJ software and normalized to the loading control result for each condition. The siRNA control result was set as 1.