Effects of ivermectin and lotilaner on Phh-GluCl receptor in X. laevis oocytes.
A. Representative current traces from a single oocyte expressing Phh-GluCl challenged with 1 mM glutamate and 10 nM ivermectin followed by 1 μM ivermectin. The bars indicate the time period of agonist application. B. Ivermectin concentration-response curve for Phh-GluCl (mean +/- SEM, n = 5 oocytes). Data were normalized to the current amplitude of a first application of 100 μM of glutamate. C. Representative current traces from oocytes perfused with 100 μM glutamate (Glu) alone (left), with 1–30 μM lotilaner prior to co-application with 100 μM Glu (middle) and after wash out (right). D. Bar chart (mean + SEM, n = 5) of the response elicited by co-application of 100 μM glutamate with 1–30 μM lotilaner normalized to and compared with the response to 100 μM glutamate alone (One-way ANOVA with Tukey’s Multiple Comparisons Test, NS: Not Significative).