Effects of LFE on hepatic steatosis in ob/ob mice.

After 8 weeks of LFE administration, plasma levels of ALT and AST, and TG were determined using commercial kits (A). Liver tissues were frozen and tissue sections were stained with either H&E or Oil Red O, and visualized under an optical microscope (B). Hepatic TG contents were measured using commercial kit (C). Plasma levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured using ELISA kits (D). Hepatic mRNA levels of IL-1β and IL-6 were measured (E). *P<0.05, **P<0.01, ***P<0.001 vs. vehicle (0.05% CMC).