Effects of IL-1β, tumor necrosis factor alpha (TNF-α) and IL-17 used alone and in combination on c-mRNA expression
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posted on 2011-12-31, 04:37 authored by Corinne Granet, Wova Maslinski, Pierre MiossecCopyright information:
Taken from "Increased AP-1 and NF-κB activation and recruitment with the combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocytes"
Arthritis Research & Therapy 2004;6(3):R190-R198.
Published online 26 Feb 2004
PMCID:PMC416439.
Copyright © 2004 Granet et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
After 12 hours of culture without FCS, rheumatoid arthritis fibroblast-like synoviocytes were stimulated for 20 min with individual or combined cytokines at low concentrations: IL-1 (10 pg/ml), TNF-α (10 pg/ml) and IL-17 (50 ng/ml). Cells were lyzed and total mRNA was extracted. RT-PCR was performed as described in Materials and methods. The intensity of the respective and c-bands was measured by densitometry. Results were expressed as the ratio between c-values divided by those of the internal control and were the mean of three independent experiments. CT, control.