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Effect of the Idefix 5′ UTR and/or LTR on the nuclear localization of neighbouring chromosomal DNA

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posted on 31.12.2011, 07:18 by E. Brasset, F. Bantignies, F. Court, S. Cheresiz, C. Conte, C. Vaury

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Taken from "Idefix insulator activity can be modulated by nearby regulatory elements"

Nucleic Acids Research 2007;35(8):2661-2670.

Published online 10 Apr 2007

PMCID:PMC1885662.

© 2007 The Author(s)

() FISH-immunostaining in interphase nuclei of larval eye imaginal discs. Single slices of individual nuclei showing characteristic examples of data obtained in different lines are presented. The FISH signals of the 82E8 and 86F6 loci containing the p2L and p2LU transgenes, respectively (red), and nuclear lamin (green), are shown. Arrows indicate cases of localization of the locus at the nuclear lamin. Genotypes are indicated by −2L or −2LU for the absence of the corresponding transgene at the indicated locus, or by +2L or +2LU for the presence of it. () Histograms showing the 3D position of each locus hybridization signal with (black bars) or without (white bars) the respective transgenes. The relative sample frequency is plotted against the FISH spot-to-lamin distance (expressed as a% of the nuclear radius). Three larval discs were analysed for each locus. For each histogram, data from two independent loci were pooled together. () Position (means ± SEM) of each locus, either with (black squares) or without (white squares) the respective transgene, relative to the nuclear radius.

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