Effect of supplemental Met at 0, 125, 250, and 500 μM on relative histone methylation at 24 h post-treatment in bovine mammary alveolar epithelial cells (MACT).

Relative histone methylation in lysine residue 9 (K9) or lysine residue 27 (K27) was measured through a fluorescent resonance energy transfer. A priori orthogonal contrasts were analyzed in order to determine linear (L) and quadratic (Q) effects over Met supplementation. Values are means, with standard errors represented by vertical bars.