Effect of hydrogen peroxide (H2O2) on porcine myoblast viability.
After 48 h in growth medium, cultured myoblasts were treated with H2O2 (0, 0.1, 0.2, 0.3, 0.5 and 1.0 mM) for 30 min, 1 h or 2 h and viability was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS assay). Each H2O2 treatment was compared to the 0 mM H2O2 control, which was given a value of 1. The 0.3 mM H2O2 and 1 h condition (circle) was selected for all H2O2-induced oxidative stress analyses. Results are presented as mean absorbance values of triplicate from four independent experiments. SEM at 30 min = 0.01, 1 h = 0.01, 2 h = 0.01. †0.05<P<0.1; ***P≤0.001.