pone.0241390.g003.tif (6.37 MB)

Effect of different concentration of ROCK and TGFβRI inhibitors on smooth muscle α-actin fibers.

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posted on 26.10.2020, 18:29 by George Gilles, Andrew D. McCulloch, Cord H. Brakebusch, Kate M. Herum

A) Schematic diagram (modified from Herum et al., JCM, 2017) of the three main mechanically activated signalling pathways that lead to myofibroblast differentiation and pro-fibrotic gene expression. B) Inhibition points using the inhibitors Y27632, SB431542 and cyclosporine A (CsA). Immunostaining of cardiac fibroblasts for smooth muscle α-actin (SMA) in cardiac fibroblasts treated with different concentrations of Y27632 (C) and SB431542 (D and E). The micrograph in E is the same as in D. Phalloidin was used to stain F-actin (C) and DAPI to stain nuclei (D and E). Scale bar 100μm (C) and 200μm (D and E).