Effect of different concentration of ROCK and TGFβRI inhibitors on smooth muscle α-actin fibers.
A) Schematic diagram (modified from Herum et al., JCM, 2017) of the three main mechanically activated signalling pathways that lead to myofibroblast differentiation and pro-fibrotic gene expression. B) Inhibition points using the inhibitors Y27632, SB431542 and cyclosporine A (CsA). Immunostaining of cardiac fibroblasts for smooth muscle α-actin (SMA) in cardiac fibroblasts treated with different concentrations of Y27632 (C) and SB431542 (D and E). The micrograph in E is the same as in D. Phalloidin was used to stain F-actin (C) and DAPI to stain nuclei (D and E). Scale bar 100μm (C) and 200μm (D and E).