pone.0239496.g004.tif (196.02 kB)

Effect of carnosine on the accumulation of intracellular reactive oxygen species (ROS) and protein carbonyls in porcine myoblasts.

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posted on 18.09.2020, 17:30 by Marie-France Palin, Jérôme Lapointe, Claude Gariépy, Danièle Beaudry, Claudia Kalbe

(A, C) Effect of carnosine treatment (0, 10, 25 and 50 mM, 48 h) without H2O2 (-H2O2). The mean values of each carnosine concentration were compared to the carnosine 0 mM treatment using a Dunnett test. (B, D) Effect of carnosine pre-treatment (0, 10, 25 and 50 mM, 48 h) before H2O2-induced oxidative stress (0.3 mM, 1 h; + H2O2). Ctr. = no carnosine and no H2O2. The effect of H2O2 on ROS and protein carbonyls accumulation was determined by specific contrast analyses between the Ctr. and 0 mM carnosine + H2O2 treatments (horizontal bars). The effect of carnosine in the presence of an H2O2-induced oxidative stress was determined by comparing each dose of carnosine to the carnosine 0 mM + H2O2 treatment (Dunnett test). Values correspond to means ± SEM of n = 4 independent experiments in duplicate. ROS SEM without H2O2 = 0.22 (A) and with H2O2 = 0.17 (B). Protein carbonyls SEM without H2O2 = 0.12 (C) and with H2O2 = 0.11 (D). 0.05<P<0.1; ***P≤0.001.