Effect of carnosine on the accumulation of intracellular reactive oxygen species (ROS) and protein carbonyls in porcine myoblasts.
(A, C) Effect of carnosine treatment (0, 10, 25 and 50 mM, 48 h) without H2O2 (-H2O2). The mean values of each carnosine concentration were compared to the carnosine 0 mM treatment using a Dunnett test. (B, D) Effect of carnosine pre-treatment (0, 10, 25 and 50 mM, 48 h) before H2O2-induced oxidative stress (0.3 mM, 1 h; + H2O2). Ctr. = no carnosine and no H2O2. The effect of H2O2 on ROS and protein carbonyls accumulation was determined by specific contrast analyses between the Ctr. and 0 mM carnosine + H2O2 treatments (horizontal bars). The effect of carnosine in the presence of an H2O2-induced oxidative stress was determined by comparing each dose of carnosine to the carnosine 0 mM + H2O2 treatment (Dunnett test). Values correspond to means ± SEM of n = 4 independent experiments in duplicate. ROS SEM without H2O2 = 0.22 (A) and with H2O2 = 0.17 (B). Protein carbonyls SEM without H2O2 = 0.12 (C) and with H2O2 = 0.11 (D). †0.05<P<0.1; ***P≤0.001.