pone.0239496.g003.tif (262.46 kB)

Effect of carnosine on porcine myoblast viability (A, B) and proliferation (C, D).

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posted on 18.09.2020, 17:30 by Marie-France Palin, Jérôme Lapointe, Claude Gariépy, Danièle Beaudry, Claudia Kalbe

(A, C) Effect of carnosine treatment (0, 10, 25 and 50 mM, 48 h) without H2O2 (-H2O2). The mean values of each carnosine concentration were compared to the 0 mM carnosine treatment using a Dunnett test. (B, D) Effect of carnosine pre-treatment (0, 10, 25 and 50 mM, 48 h) before H2O2-induced oxidative stress (0.3 mM, 1 h; + H2O2). The effect of carnosine in the presence of an H2O2-induced oxidative stress was determined by comparing each dose of carnosine to the 0 mM carnosine+ H2O2 treatment (Dunnett test). MTS = 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay; BRDU = Bromodeoxyuridine (BRDU) assay. Values correspond to means ± SEM of n = 4 independent experiments in triplicates. MTS SEM without H2O2 = 0.02 (A) and with H2O2 = 0.01 (B). BRDU SEM without H2O2 = 0.02 (C) and with H2O2 = 0.01 (D). **P≤0.01; ***P≤0.001.