Effect of a 5-day treatment with morusin B (in mg/kg) on the activity of matrix metalloproteinases in TNBS-induced colitis, compared to the intact group, the untreated group (CTRL), and the group treated with sulfasalazine 50 mg/kg (SAS).
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(A) MMP9 activity, (B) total MMP2 activity, (C) activity of the pro-form of MMP2, (D) activity of the mature form of MMP2, (E) pro-MMP2/MMP2 ratio. The activity of MMPs was detected by zymography, quantified by densitometry, and normalized to a standard control (1% FBS). A representative zymogram is shown (F). The results are expressed as the mean, with error bars representing SEM. CTRL vs. intact group: ## p < 0.01, ### p < 0.001, #### p < 0.0001; CTRL vs. treated groups: ** p < 0.01, *** p < 0.001, **** p < 0.0001.