pone.0167058.g002.tif (519.18 kB)

Effect of RvD2 on TNF-α-induced reactivity and inflammation in human bronchi.

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posted on 09.12.2016 by Rayan Khaddaj-Mallat, Chantal Sirois, Marco Sirois, Edmond Rizcallah, Sofia Marouan, Caroline Morin, Éric Rousseau

a. Bar graph of the contractile activity induced by bronchoactive agents (MCh, His and U-46619) on 48-h cultured human bronchi in control (untreated, n = 7–12) conditions, 10 ng/ml TNF-α (n = 14–19), TNF-α + 300 nM RvD2 (n = 15–26), TNF-α + RvD2 + 300 nM WRW4 (n = 11–14) or TNF-α + RvD2 + 1 μM WRW4 (n = 10–11), *P < 0.05. b. Western blot analyses using specific antibodies against P-p38-MAPK and total p38-MAPK. Staining densities of P-p38-MAPK are expressed as a function of p38-MAPK levels (n = 5, * P < 0.05). c. Western blot analyses were performed using antibodies against p-c-Fos and total c-Fos. The relative density ratio of p-c-Fos to total c-Fos was used to quantify the comparative effects of RvD2 on TNF-α-pretreated human bronchi (n = 5, * P < 0.05). d. Western blot analyses were performed using antibodies against p-c-Jun and total c-Jun. Staining densities of p-c-Jun are expressed as a function of c-Jun levels (n = 5, * P < 0.05).

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