Effect of RvD2 on TNF-α-induced reactivity and inflammation in human bronchi.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
a. Bar graph of the contractile activity induced by bronchoactive agents (MCh, His and U-46619) on 48-h cultured human bronchi in control (untreated, n = 7–12) conditions, 10 ng/ml TNF-α (n = 14–19), TNF-α + 300 nM RvD2 (n = 15–26), TNF-α + RvD2 + 300 nM WRW4 (n = 11–14) or TNF-α + RvD2 + 1 μM WRW4 (n = 10–11), *P < 0.05. b. Western blot analyses using specific antibodies against P-p38-MAPK and total p38-MAPK. Staining densities of P-p38-MAPK are expressed as a function of p38-MAPK levels (n = 5, * P < 0.05). c. Western blot analyses were performed using antibodies against p-c-Fos and total c-Fos. The relative density ratio of p-c-Fos to total c-Fos was used to quantify the comparative effects of RvD2 on TNF-α-pretreated human bronchi (n = 5, * P < 0.05). d. Western blot analyses were performed using antibodies against p-c-Jun and total c-Jun. Staining densities of p-c-Jun are expressed as a function of c-Jun levels (n = 5, * P < 0.05).