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Ectopic EWSR1-FLI1 interacts with specific DNA and protein-binding sites.

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posted on 05.06.2020, 17:34 authored by Daniel J. García-Domínguez, Lourdes Hontecillas-Prieto, Eduardo Andrés León, Sara Sánchez-Molina, Pablo Rodríguez-Núñez, Francisco J. Morón, Nabil Hajji, Carlos Mackintosh, Enrique de Álava

A. EWSR1-FLI1 immunoprecipitation using anti-FLAG antibody in parental HeLa3G cells (control) and two different clones (HeLa Tet-On EF #3.10 and #3.15) in the presence or absence of doxycycline. B. Binding of EWSR1-FLI1 to the CAV1 enhancer and the promoters of the CAV1, EZH2 and AURKB genes. qPCR analysis of EWSR1-FLI1 ChIP signals for HeLa Tet-On EF #3.15 clone. C. Co-immunoprecipitation of EWSR1-FLI1 3×FLAG C-terminal and LSD1 protein in HeLa Tet-On EF #3.15 clone.