EPR spectra and quantification of detected from zebrafish adult heart tissue using CMH under rotenone and PEG-SOD treated conditions.
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posted on 2025-02-21, 18:47 authored by Mitra Sabetghadam Moghadam, Eli Wiens, Sébastien Gauvrit, Ramaswami Sammynaiken, Michelle M CollinsRepresentative EPR spectra are shown for rotenone-treated adult heart (dashed line; A), and rotenone + PEG-SOD treated heart (solid line; A). Quantification of normalized EPR signal amplitude (B). A 1 mM CMH concentration was used for all conditions; n=3 replicates/adult hearts. PEG-SOD, polyethylene glycol-conjugated superoxide dismutase; Rot, rotenone. Error bar corresponds to SEM.
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nitrone radical adductsgenerate stable adductselectron paramagnetic resonancebiologically relevant radicalsmethod commonly employeddiv >< pzebrafish pathologies linkeddetecting free radicalszebrafish cmh spin probefree radicalsspin trappingreliable methodcmh probezebrafish heartstetramethyl pyrrolidinesuperoxide (),pyrroline noxidative stressoptimized protocolextremely shortexcellent choicedetected directlydata suggestbiological samplesappropriate compound
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