Dynamic location of Rab11B between the golgi and the nascent IMC during cell division.

(A) Immunofluorescent analysis of parasites expressing the wild type Rab11B under control of the endogenous promoter (pRab11B_wt). Parasites were double-labelled with anti-myc (red) and anti-IMC1 (green) to visualise Rab11B and IMC1. Rab11B cycles from a location close to the nucleus (interphase parasites in upper panel) to the growing IMC of the daughter parasites (dividing parasites in middle and lower panel) in a cell cycle dependent manner. (B) Immunofluorescent analysis of parasites expressing ddRab11B_wt and the Golgi marker (GRASP-RFP in red) in presence of 0.1 µM Shld-1 for 24 h. Parasites are labelled with anti-myc antibody (green). Rab11B localises to the Golgi at the initial phase of cell division (upper panel and inlet) and accumulates to the nascent IMC of daughter parasites during endodyogeny (see A,C middle panel). After endodyogeny is completed Rab11B again accumulates at the Golgi (lower panel and inlet). (C) Immunofluorescent analysis of parasites expressing the ddRab11B_wt and the α-tubulin marker (mCherry-α-Tubulin in red) in presence of 0.25 µM Shld-1 for 24 h. Parasites are labelled with anti-myc antibody (green). At the onset of endodyogeny (upper panel) Rab11B accumulates to the newly assembled conoid of the daughter cells. Later becomes concentrated along the daughter scaffold throughout endodyogeny (middle and lower panel). Scale bars represent 5 µm.