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Dose-response relationship of staurosporine and NEM on KCC2 transport activity.

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posted on 15.05.2020, 17:37 by Jinwei Zhang, Antje Cordshagen, Igor Medina, Hans Gerd Nothwang, Jacek R. Wisniewski, Michael Winklhofer, Anna-Maria Hartmann

HEKrnKCC2b cells were treated with different concentrations of staurosporine (5–80 μM; A) or NEM (25–3,000 μM; B) for 15 min. Transport activity of rnKCC2b was determined by Tl+ flux measurements. Treatment with staurosporine resulted in a classical Michaelis-Menden curve, whereas NEM treatment showed a sigmoidal curve with a Hill coefficient of 5.2 ± 1.3 indicating a positive cooperative effect on rnKCC2b. The graph represents the data of at least five independent measurements (each consisting of three technical replicates) normalized to untreated HEKrnKCC2b.