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Distribution of single and complex chimeras.

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posted on 11.03.2016, 01:01 by Maria E. Morales, Rebecca S. Derbes, Catherine M. Ade, Jonathan C. Ortego, Jeremy Stark, Prescott L. Deininger, Astrid M. Roy-Engel

Alu-Alu recombination can generate two types of outcomes: simple chimera or complex chimera. A. A simple chimera shows only one recombination site (left portion of the Alu sequence chimera derived from Alu1 and right portion derived from Alu2). B. A complex chimera shows multiple recombination sites with intermixed regions of the chimera derived from the two Alu sequences. Schematic of actual recovered outcomes are shown below the diagrams. C. The 5 and 15%AARP HEK 293 cell line was incubated with media containing 100 μM NiCl2. Untreated cells were used as the reference control. Sequence analyses of the recovered events showed that two types of Alu-Alu recombination products can be observed: simple and complex. NAR events recovered from the nickel treated cells were further classified as single (gray) or complex chimeras (white). The results are expressed as mean ± SD of three independent experiments. Statistical differences are indicated as *P<0.05; (Fisher’s exact test); ns = not significant. Numbers above each column represent the total colonies analyzed; sequence alignments are shown in S7 Fig.