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Dimethylated histone H3/K4 association with 5′-flanking region is correlated with IL-2 inducibility in immature thymocytes: ChIP analysis of the locus in thymocytes from SCID and MHC-deficient mice

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posted on 31.12.2011, 00:01 by Satoko Adachi, Ellen V. Rothenberg

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Taken from "Cell-type-specific epigenetic marking of the gene at a distal -regulatory region in competent, nontranscribing T-cells"

Nucleic Acids Research 2005;33(10):3200-3210.

Published online 3 Jun 2005


© The Author 2005. Published by Oxford University Press. All rights reserved

Cells from MHC-deficient mice, >95% CD4 CD8 TCR-low, were cultured with or without PMA/A23187 for 3 h. Cells from SCID mice, >95% CD4 CD8 TCR-negative, were cultured with or without stimulation for 2 h, and IL-1α was also added as indicated. To correct for differences in efficiency of immune precipitation between these samples due to the extensive differences in cell physiology, ChIP analysis results for diMeH3/K4 at the locus for each sample were normalized by results at the S16 ribosomal protein locus. Two independent experiments were done with similar results.