Combined forces of hydrostatic pressure and actin polymerization drive endothelial tip cell migration and sprouting angiogenesis.
Raw image files used to generate figures in Kondrychyn et al., Combined forces of hydrostatic pressure and actin polymerization drive endohtelial tip cell migration and sprouting angiogenesis. https://doi.org/10.7554/eLife.98612.2 (revised version)
Fig. 1: Raw image files showing the expression of aqp1a.1 and aqp8a.1 mRNA in developing intersegmental vessels as detected by RNAscope in 20 and 22 hpf zebrafish embryos. These files were used to generate Figures 1A and E in the manuscript:
Figure 1A: channel C=0: all nuclei (DAPI), channel C=1: endothelial nuclei (H2b-EGFP), channel C=2: aqp1a.1 mRNA, channel C=3: aqp8a.1 mRNA
Figure 1E: channel C=0: nuclei (DAPI), channel C=1: apq8a.1 mRNA, channel C=2: aqp1a.1 mRNA
Fig. 2: Raw images showing the localisation of Aqp1a.1-mEmerald and Aqp8a.1-mEmerald in endothelial tip cells during the formation of intersegmental vessel formation. These files were used to generate Figures 2A and B.
Figure 2A: channel C=0: Aqp1a.1-mEmerald, channel C=1: mCherry-CAAX
Figure 2B: channel C=0: Aqp8a.1-mEmerald, channel C=1: mCherry-CAAX
Fig. 3: Raw images used to analyse trunk vasculature of aqp1a.1, aqp8a.1 and aqp1a.1;aqp8a.1 mutant zebrafish. These files were used to generate Figures 3A, B C, D G, H, I and J.
Channel C=0: GFP, Channel C=1: mCherry
MIPs of images 3A-D and I-J were stitched as tail1+tail2 to make the final images.
Fig. 4: Raw images used to examine endothelial tip cell protrusion and migration in aqp1a.1;aqp8a.1 double mutant zebrafish in Figures 4D - G.
Channel C=0: GFP,Channel C=1: mCherry
Figure 4D: second vessel from the right was used for illustration (7 min time interval)
Figure 4E: fourth vessel from the right was used for illustration (7 min time interval)
Fig. 5: Raw images used to analyse volume of wildtype, aqp1a.1;aqp8a.1 double mutant and aqp1a.1-overexpressing endothelial tip cell in Figures 5A - C.
Channel C=0: mEmerald or EGFP; Channel C=1: mCherry
Fig. 6: Raw images used to examine the expression pattern of lrrc8aa mRNA expression and the effects of SWELL1 inhibition on intersegmental vessel formation and tip cell behaviour in Figures 6C, F, G, M and N.
Figure 6C: channel C=0: nuclei (DAPI), channel C=1: lrrc8aa mRNA, channel C=2: aqp1a.1 mRNA, channel C=3: aqp8a.1 mRNA
Figures 6F and G: channel C=0: GFP, channel C=1: mCherry
Figure 6M: second vessel from the left was used for illustration (5 min time interval). Channel C=0: GFP, channel C=1: mCherry
Figure 6N: third vessel from the left was used for illustration (5 min time interval). Channel C=0: GFP, channel C=1: mCherry
Fig. 7: Raw image files used to examine the combined effects of actin polymerization and hydrostatic pressure in sprouting angiogenesis in Figures 7A - D.
channel C=0: GFP, channel C=1: mCherry
