Determining the role of STAT-3 signaling in pom-induced increases in ICAM-1 and B7-2.
(A) Surface expression levels of ICAM-1 and B7-2 in BCBL-1 cells 48 hours after treatment with DMSO ctrl or STAT3 inhibitor S3I-201 (10 and 100μM). (B) BCBL-1 cells were pretreated with S3I-201 for approximately 1 hour and then treated with 1μM Pom for another 48 hours. Histograms show ICAM-1 and B7-2 levels in the presence of 1μM Pom alone or in combination with S3I-201. Cells were stained with PerCP/Cy5.5-conjugated antibodies and analyzed using flow cytometry.